Carbohydrate analysis by gas chromatography. (a) Dried nematode A. avenae extract containing glucose and accumulated trehalose. (b) Dried rotifer P. roseola extract containing glucose, but no trehalose or other disaccharide. Glucose is represented by the doublet of peaks emerging at 7 min, while trehalose gives a single peak at 13 min. From Lapinski & Tunnacliffe (2003); Caprioli et al. (2004) and McGee (2006) have similar findings with other bdelloid species.