skip to primary navigationskip to content
 

images

yeast.007

 
yeast

Survival of air drying (16 h, 30˚C) of wild type and mutant yeast strains at various stages of growth (left panel) and corresponding intracellular trehalose concentrations (right panel)

spliceleaderbig

 
spliceleader

Fig.1: Bdelloid spliced leader RNA, which donates the SL exon to mRNA precursors by trans-splicing

sciencerotifer

 
sciencefig3big

 
sciencefig3

Bdelloid LEA protein molecular shield assay. Citrate synthase (CS), with or without LEA proteins or BSA, and the latter proteins alone where indicated, were subjected to two cycles of vacuum drying and rehydration. Light scattering by protein particulates was measured by apparent absorption at 340 nm in the spectrophotometer. Error bars show standard deviation (n = 3); ns, not significantly different (P > 0.05); significant values *P < 0.05 or **P < 0.001.

science

 
podcasticonbig

 
podcasticon

 
pnas

 
phosphobig

 
phospho

JNK and p38 MAPK activation in T-REx 293 cells by desiccation (Huang and Tunnacliffe, 2004).

philodinabig

 
philodina

The bdelloid rotifer Philodina roseola

pdficonbig

 
pdficon

 
p. superbus livedeadbig

 
p. superbus livedead

Fig. 2: Live/dead staining of P. superbus cell cultures.

leatree

 
Learotifer

Bdelloid rotifer A. ricciae with DNA stained blue and LEA protein stained red.

leafig2big

 
leafig2

Fig 2: LEA protein reduces protein aggregation in vivo. Confocal microscopy of human cells after introduction of HD polyQ ("EGFP-HDQ74") expression construct, with (induced) or without (uninduced) expression of LEA protein. Nematode LEA protein AavLEA1 is immunostained red, polyQ protein is green and nuclei are stained blue with DAPI. Indicative polyQ aggregates are shown by arrowheads; fewer aggregates are observed in the presence of LEA protein. See Chakrabortee et al. (2007) for more detail.

leafig1big

 
leafig1

Fig 1: Aggregation and activity of citrate synthase after desiccation, in the presence of nematode LEA protein or BSA. Aggregation is measured by light scattering (apparent absorbance at A340); enzyme activity is assayed according to standard methods and results expressed as percentage of control activity. One drying cycle corresponds to vacuum drying (without freezing) for 1 h followed by immediate rehydration in water to the original volume. Results after two and four drying cycles are shown. See Goyal et al. (2005) for more detail.

leacellsbig

 
leacells

Human cell line expressing a nematode LEA protein from Aphelenchus avenae.

hoekbig

 
hoek

Antony van Leeuwenhoek

graph3big

 
graph3

 
graph2big

 
graph2

 
graph1big

 
graph1

 
gfpnematodebig

 
gfpnematode

C. elegans expressing GFP (green) with DNA stained blue.

dapirotifer

 
bdelloidnotrehalosebig

 
bdelloidnotrehalose

Carbohydrate analysis by gas chromatography. (a) Dried nematode A. avenae extract containing glucose and accumulated trehalose. (b) Dried rotifer P. roseola extract containing glucose, but no trehalose or other disaccharide. Glucose is represented by the doublet of peaks emerging at 7 min, while trehalose gives a single peak at 13 min. From Lapinski & Tunnacliffe (2003); Caprioli et al. (2004) and McGee (2006) have similar findings with other bdelloid species.

alan

 
aavenae cells differentiatingbig

 
aavenae cells differentiating

Fig. 3: A. avenae cells differentiating in culture

a. avenae livedeadbig

 
a. avenae livedead

Fig. 1: Live/dead staining of A. avenae cell cultures.

RSS Feed Latest news

Alumnus Professor McKinley elected Royal Society Fellow

Apr 23, 2019

Alumnus Professor Gareth McKinley among the 51 distinguished scientists elected as new Royal Society Fellows for his exceptional contribution to science.

Dr Vassiliadis celebrates the contributions of Roger W.H. Sargent at memorial event

Apr 17, 2019

Dr Vassiliadis contributed a talk on optimal control in chemical engineering at a memorial event for Roger W.H. Sargent, founder of process systems engineering.

A chemical garden that’s also a chemical clock

Apr 12, 2019

Research from our Cardoso lab and their colleagues in Spain, published in Angewandte Chemie, combines two classic demos in one beautiful example of active fluid dynamics.

View all news

Upcoming events

CEB Research Conference 2019

Jun 25, 2019

Department of Chemical Engineering and Biotechnology, Philippa Fawcett Drive, Cambridge, CB3 0AS

Machine learning and AI in (bio)chemical engineering

Jul 08, 2019

Department of Chemical Engineering and Biotechnology, Philippa Fawcett Drive, Cambridge, CB3 0AS

Stokes200 Symposium

Sep 15, 2019

Pembroke College, Cambridge

VPF8 Viscoplastic Fluids: from Theory to Application 2019

Sep 16, 2019

Jesus College, Cambridge

Upcoming events