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Department of Chemical Engineering and Biotechnology

 

Molecular Trajectory Imaging by Eric Rees

Imaging Single Molecule Trajectories and Diffusion.  Each track on this image  is the experimentally-measured trajectory of a single fluorescent  molecule undergoing diffusion on a cell membrane. The paths are colour  coded by their observed diffusivity, from slowest (red) to fastest  (blue).  Method. The small carbocyanine dye molecule DiD was used to  stain the cell membrane of a neuroblastoma cell. The position of these  fluorescent molecules can be precisely tracked by Localisation  Microscopy. The principle of Localisation Microscopy is that the image  of a single molecule is a diffraction limited blur of light  approximately one wavelength (~500 nm) in diameter. The position of  the molecule corresponds to the centre of the blur, and this position  can be determined very precisely, to within 10-20 nm, by computational  fitting. One application of this principle is in super-resolution  imaging, in which we precisely determine fluorophore positions on a  specimen and use this information to draw a similarly precise (i.e.  with sub-wavelength resolution)

Latest news

September paper of the month: flexible production of micro and nanofluidic devices

22 October 2019

Researchers from our Laser Analytics group have developed a laser-based manufacturing process that can produce combined nanofluidic and microfluidic devices in a fast and scalable manner.

Collaborative air pollution project wins Vice-Chancellor's Research Impact and Engagement Award

15 October 2019

Student-led initiative Open-Seneca, which aims to create a global low-cost mobile air pollution sensor network driven by citizen science, was a winner in the collaboration category of the annual awards from the University's Vice-Chancellor

Sensor CDT highlights key role of sensing in sustainability at annual Sensors Day conference

15 October 2019

We celebrated the work of our EPSRC Centre for Doctoral Training in Sensor Technologies for a Healthy and Sustainable Future in two events last week, showcasing contributions from students and academic and industrial partners.