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Department of Chemical Engineering and Biotechnology

 

9. Fourier spectrum of a two-colour TIRF-SIM super-resolution reconstruction of amyloid fibrils by Florian Stroehl

Amyloid fibrils are associated with Alzheimer's disease but are too small to be imaged clearly in a conventional microscope. A clever way to nevertheless observe them is by using super-resolution imaging like TIRF-SIM. In this technique several images of the fibrils are taken under different illumination patterns, each of which provides additional bits of resolution information. These bits are then combined via a computational reconstruction process that results in the cloverleaf-like spectrum shown . To obtain the final super-resolved image of the fibrils, this spectrum must be Fourier back-transformed.

The raw data for this image was taken on the custom built TIRF-SIM microscope of the Laser Analytics Group.

Latest news

September paper of the month: flexible production of micro and nanofluidic devices

22 October 2019

Researchers from our Laser Analytics group have developed a laser-based manufacturing process that can produce combined nanofluidic and microfluidic devices in a fast and scalable manner.

Collaborative air pollution project wins Vice-Chancellor's Research Impact and Engagement Award

15 October 2019

Student-led initiative Open-Seneca, which aims to create a global low-cost mobile air pollution sensor network driven by citizen science, was a winner in the collaboration category of the annual awards from the University's Vice-Chancellor

Sensor CDT highlights key role of sensing in sustainability at annual Sensors Day conference

15 October 2019

We celebrated the work of our EPSRC Centre for Doctoral Training in Sensor Technologies for a Healthy and Sustainable Future in two events last week, showcasing contributions from students and academic and industrial partners.