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Wright O, Zhang L, Liu Y, Yoshimi T, Zheng Y, Tunnacliffe A. (2013) Critique of the use of fluorescence-based reporters in Escherichia coli as a screening tool for the identification of peptide inhibitors of Aβ42 aggregation. J Pept Sci.19 (2):74-83.


Boschetti, C., Carr, A., Crisp, A., Eyres, I., Wang-Koh, Y., Lubzens, E., Barraclough, T.G., Micklem, G., and Tunnacliffe, A. (2012) Biochemical Diversification through Foreign Gene Expression in Bdelloid Rotifers.PLoS Genet8 (11): e1003035.

Tripathi, R., Boschetti, C., McGee, B., and Tunnacliffe A. (2012) Trafficking of bdelloid rotifer late embryogenesis abundant proteins.J. Exp. Biol. 215:2786-2794.

Banton, M. C. and Tunnacliffe, A. (2012) MAPK phosphorylation is implicated in the adaptation to desiccation stress in nematodes. J. Exp. Biol.215: 4288-4298.

Chakrabortee, S., Liu, Y., Zhang, L., Matthews, H. R., Zhang, H., Pan, N., Cheng, C.-R., Guan, S-H., Guo, D.-A., Huang, Z., Zheng, Y. and Tunnacliffe, A. (2012) Macromolecular and small molecule modulation of intracellular Ab42 aggregation and associated toxicity.Biochem. J.442: 507-515.

Chakrabortee, S., Tripathi, R., Watson, M., Kaminski Schierle, G. S., Kurniawan, D. P., Kaminski, C. F., Wise, M. J.** and Tunnacliffe, A.** (2012) Intrinsically disordered proteins as molecular shields. Mol. BioSyst. 8: 210-219.

Eyres, E., Frangedakis, E., Fontaneto, D., Herniou, E. A., Boschetti, C., Carr, A., Micklem, G., Tunnacliffe, A. and Barraclough, T. G. (2012) Multiple functionally divergent and conserved copies of alpha tubulin in bdelloid rotifers. BMC Evol. Biol. 12: 148.

Furuki, T., Shimizu, T., Chakrabortee, S., Yamakawa, K., Hatanaka, R., Takahashi, T., Kikawada, T., Okuda, T., Mihara, H., Tunnacliffe, A. and Sakurai, M. (2012) Effects of Group 3 LEA protein model peptides on desiccation-induced protein aggregation. Biochim. Biophys. Acta 1824: 891-897.

Warner, A. H., Chakrabortee, S., Tunnacliffe, A. and Clegg, J. S. (2012) Complexity of the LEA proteome in Artemia species. Comp. Biochem. Phys. D 7: 260-267.

Wu, H.-Q., Zhang, L., Zhang, F., Meng, N.-Y., He, Z.-D., Tunnacliffe, A. and Zheng, Y.-Z. (2012) Research on the suppression of Aβ42 aggregation activity by extracts of food and drug Chinese medicine. China J. Tradit. Chinese Med. Pharm. 27: 2199-2202.

Wright, O.*, Yoshimi, T.* and Tunnacliffe, A. (2012) Recombinant production of cathelicidin-derived antimicrobial peptides in Escherichia coli using an inducible autocleaving enzyme tag. New Biotechnol. 29: 352-358.

Zhang, H., Pan, N., Zou, S., Xiao, L., Cao, Z., Tunnacliffe, A. and Huang, Z. (2012) Inhibition of polyglutamine-mediated proteotoxicity by Astragalus membranaceus polysaccharide through DAF-16/FOXO transcription factor in Caenorhabditis elegans. Biochem. J. 441: 417-424


Boschetti, C., Pouchkina-Stantcheva, N. N., Hoffman, P., and Tunnacliffe, A. (2011) Foreign genes and novel hydrophilic protein genes participate in the desiccation response of the bdelloid rotifer Adineta ricciae. J. Exp. Biol. 214:59-68.

Liu, Y.*, Chakrabortee, S.*, Li, R., Zheng, Y. and Tunnacliffe, A. (2011) Both plant and animal LEA proteins act as kinetic stabilisers of polyglutamine-dependent protein aggregation. FEBS Lett. 585: 630-634.

Ratnakumar, S., Hesketh, A., Gkargkas, K., Wilson, M., Rash, B. M., Hayes, A., Tunnacliffe, A. and Oliver, S. G. (2011) Phenomic and transcriptomic analyses reveal that autophagy plays a major role in desiccation tolerance in Saccharomyces cerevisiae. Mol. Biosyst. 7:139-149.


Chakrabortee, S., Meersman, F., Kaminski Schierle, G. S., Bertoncini, C. W., McGee, B., Kaminski, C. F. and Tunnacliffe, A. (2010) Catalytic and chaperone-like functions in an intrinsically disordered protein associated with desiccation tolerance. Proc. Natl. Acad. Sci. USA 107:16084-16089.

Huang, Z. Banton, M. C. and Tunnacliffe, A. (2010) Modeling anhydrobiosis: activation of the mitogen-activated protein kinase ERK by dehydration in both human cells and nematodes. J. Exp. Zool. 313A:660-670.

Narváez-Reinaldo, J. J., Barba, I., González-López, J., Tunnacliffe, A., and Manzanera, M. (2010) Rapid Method for Isolation of Desiccation-Tolerant Strains and Xeroprotectants. Appl. Environ. Microbiol. 76:5254-5262.

Reardon, W., Chakrabortee, S., Pereira, T. C., Tyson, T., Banton, M. C., Dolan, K. M., Culleton, B. A., Wise M. J., Burnell A. M., and Tunnacliffe, A. (2010) Expression profiling and cross-species RNA interference (RNAi) of desiccation-induced transcripts in the anhydrobiotic nematode Aphelenchus avenae. BMC Mol. Biol. 11:6.

Wang, X. J., Boschetti, C., Ruedas-Rama, M. J., Tunnacliffe, A. and Hall, E. A. H. (2010) Ratiometric pH-dot ANSors. Analyst 135:1585-1591.

Bahrndorff, S., Tunnacliffe, A., Wise, M. J., McGee, B., Holmstrup, M. and Loeschcke, V. (2009) Bioinformatics and protein expression analyses implicate LEA proteins in the drought response of Collembola. J. Insect Physiol. 55:210-217.

Vilchez, S., Tunnacliffe, A. and Manzanera, M. (2008) Tolerance of plastic-encapsulated Pseudomonas putidaKT2440 to chemical stress. Extremophiles. 12:297-299.

Chakrabortee, S., Boschetti, C., Walton, L. J., Sarkar, S. Rubinsztein, D. C. and Tunnacliffe, A. (2007) Hydrophilic protein associated with desiccationtolerance exhibits broad protein stabilization function. Proc. Natl. Acad. Sci. USA 104:18073-18078.

Pouchkina-Stantcheva, N. N., McGee, B. M., Boschetti, C., Tolleter, D., Chakrabortee, S., Popova, A. V., Meersman, F., Macherel, D., Hincha, D. K. and Tunnacliffe, A. (2007) Functional divergence of former alleles in an ancient asexual invertebrate. Science 318: 268-271.

Sarkar, S., Davies, J. E., Huang, Z., Tunnacliffe, A. and Rubinsztein, D. C. (2007) Trehalose, a novel mTOR-independent autophagy enhancer, accelerates the clearance of mutant huntingtin and α-synuclein. J. Biol. Chem. 282:5641-5652.

Kikawada, T., Nakahara, Y., Kanamori, Y., Iwata, K.-I., Watanabe, M., McGee, B., Tunnacliffe, A. and Okuda, T. (2006) Dehydration-induced expression of LEA proteins in an anhydrobiotic chironomid. Biochem. Biophys. Res. Comm. 348:56-61.

Ratnakumar, S. and Tunnacliffe, A. (2006) Intracellular trehalose is neither necessary nor sufficient for desiccation tolerance in yeast. FEMS Yeast Res. 6:902-913.

Goyal, K., Pinelli, C., Maslen, S. L., Rastogi, R. K., Stephens, E. and Tunnacliffe, A. (2005) Dehydration-regulated processing of late embryogenesis abundant protein in a desiccation-tolerant nematode. FEBS Lett. 579:4093-4098.

Goyal, K., Browne, J. A., Burnell, A. M. and Tunnacliffe, A. (2005) Dehydration-induced tps gene transcripts from an anhydrobioticnematode contain novel spliced leaders and encode atypical GT-20 familyproteins. Biochimie 87:565-574.

Huang, Z. and Tunnacliffe, A. (2005) Gene induction by desiccation stress in human cell cultures. FEBS Lett. 579:4973–4977.

Pouchkina-Stantcheva, N. N. and Tunnacliffe, A. (2005) Spliced-leader RNA mediated trans-splicing in phylum Rotifera. Mol. Biol. Evol. 22:1482-1489.

Goyal, K., Walton, L. J. and Tunnacliffe, A. (2005) LEA proteins prevent protein aggregation due to water stress. Biochem. J. 388:151-157.

Browne, J. A., Dolan, K. M., Tyson, T., Goyal, K., Tunnacliffe, A. and Burnell, A. M. (2004) Dehydration-specific induction of hydrophilic protein genes in the anhydrobiotic nematode Aphelenchus avenae. Eukaryot. Cell 3:966-975.

Huang, Z. and Tunnacliffe, A. (2004) Response of human cells to desiccation: comparison with hyperosmotic response. J. Physiol. 558:181-191.

Manzanera, M., Vilchez, S. and Tunnacliffe, A. (2004) Plastic encapsulation of stabilised Escherichia coliand Pseudomonas putida. Appl. Environ. Microbiol. 70:3143-3145.

Manzanera, M., Vilchez, S. and Tunnacliffe, A. (2004) High survival and stability rates of Escherichia colidried in hydroxyectoine. FEMS Microbiol. Lett. 233:347-352.

Goyal, K., Tisi, L., Basran, A., Browne, J., Burnell, A., Zurdo, J. and Tunnacliffe, A. (2003) Transition from natively unfolded to folded state induced by desiccation in an anhydrobiotic nematode protein. J. Biol. Chem. 278:12977-12984.

Lapinski, J. and Tunnacliffe, A. (2003) Anhydrobiosis without trehalose in bdelloid rotifers. FEBS Lett. 553:387-390.

Lapinski, J. and Tunnacliffe, A. (2003) Reduction of suspended biomass in municipal wastewater using bdelloid rotifers. Water Res. 37:2027-2034.

Browne, J., Tunnacliffe, A. and Burnell, A. (2002) Plant desiccation gene found in a nematode. Nature 416:38.

Howells, A. M., Bullifent, H. L., Dhaliwal, K., Griffin, K., García de Castro, A., Frith, G., Tunnacliffe, A. and Titball, R. W. (2002) Role of trehalose biosynthesis in environmental survival and virulence of Salmonella entericaserovar typhimurium. Res. Microbiol. 153:281-287.

Manzanera, M., García de Castro, A., Tøndervik, A., Rayner-Brandes, M., Strøm, A. R. and Tunnacliffe, A. (2002) Hydroxyectoine is superior to trehalose for anhydrobiotic engineering of Pseudomonas putidaKT2440. Appl. Environ. Microbiol. 68:4328-4333.

Yamamoto, T.-A. M., Gerdes, K. and Tunnacliffe, A. (2002) Bacterial toxin RelE induces apoptosis in human cells. FEBS Lett. 519:191-194.

Bullifent, H. L., Dhaliwal, K., Howells, A. M., Goan, K., Griffin, K., Lindsay, C. D., Tunnacliffe, A. and Titball, R. W. (2000) Stabilisation of Salmonellavaccine vectors by the induction of trehalose biosynthesis. Vaccine 19:1239-1245.

García de Castro, A. and Tunnacliffe, A. (2000) Intracellular trehalose improves osmotolerance but not desiccation tolerance in mammalian cells. FEBS Lett. 487:199-202.

García de Castro, A., Bredholt, H., Strøm, A. R. and Tunnacliffe, A. (2000) Anhydrobiotic engineering of gram-negative bacteria. Appl. Environ. Microbiol. 66:4142-4144.

García de Castro, A., Lapinski, J. and Tunnacliffe, A. (2000) Anhydrobiotic engineering. Nature Biotech. 18:473.


Burnell, A. and Tunnacliffe, A. (2011) Gene induction and desiccation stress in nematodes. In Molecular and Physiological Basis of Nematode Survival, eds. Perry, R. N. and Wharton, D. CABI Publishing, Wallingford, UK.

Tunnacliffe, A., Hincha, D. K., Leprince, O. and Macherel, D. (2010) LEA proteins: versatility of form and function. In Dormancy and Resistance in Harsh Environments, eds. Lubzens, E., Cerda, J. and Clark, M. S. Topics in Current Genetics, Springer, Berlin, pp. 91-108.

Tunnacliffe, A. and Wise, M. J. (2007) The continuing conundrum of the LEA proteins. Naturwissenschaften 94:791-812.

Huang, Z. and Tunnacliffe, A. (2007) Desiccation of mammalian cells: anhydrosignaling . Methods Enzymol. 428:269-277.

Huang, Z. and Tunnacliffe, A. (2006) Cryptobiosis, aging, and cancer: yin-yang balancing of signaling networks. Rejuv. Res. 9:292-296.

Goyal,K., Walton, L. J., Browne, J. A., Burnell, A. M. and Tunnacliffe, A. (2005) Molecular anhydrobiology: identifying molecules implicated ininvertebrate anhydrobiosis. Integr. Comp. Biol. 45:702–709.

Tunnacliffe, A., Lapinski, J. and McGee, B. (2005) A putative LEA protein, but no trehalose, is present in anhydrobiotic bdelloid rotifers. Hydrobiologia 546:315-321.

Wise, M. J. and Tunnacliffe, A. (2004) POPP the question: what do LEA proteins do? Trends Plant Sci. 9:13-17.

Tunnacliffe, A. and Lapinski, J. (2003) Resurrecting van Leeuwenhoek’s rotifers: are appraisal of the role of disaccharides in anhydrobiosis. Phil. Trans. R. Soc. Lond. B, 358:1755-1771.

Tunnacliffe, A., García de Castro, A. and Manzanera, M. (2001) Anhydrobiotic engineering of bacterial and mammalian cells: is intracellular trehalose sufficient? Cryobiology 43:124-132.